New method speeds variant tracking from weeks to hours

September 21, 2021

New method speeds variant tracking from weeks to hours

UW School of Medicine Microbiology Professor Evgeni Sokurenko is drawing a line in the efforts to identify and track variants of the COVID-19 virus.

Alongside Seattle-based ID Genomics, which Sokurenko founded, the professor’s laboratory is leading the creation and future implementation of a new method to “fingerprint” all currently known variants. The method identifies the presence of dozens of mutations at once and requires only three ingredients: a dipstick, a common thermocycler instrument, and a processed test sample.

Upon coming into contact with the sample, the dipstick records which variant is present by exposing a series of lines, or bands. Every known variant of interest and concern has a correlating band pattern, which the test administrator can match to the sample.

“It's a rapid test. We can identify the variant within two hours of the sample being in hand,” says Sokurenko.

Dr. Evgeni Sokurenko and research scientist Lydia Larson prepare a rapid COVID-19 variant test.

Currently the definitive method of tracking variants of the virus, including the delta variant, is done through genomic sequencing. The process is thorough but can take up to two weeks to collect a sufficiently large batch of samples and yield results. Sokurenko’s method provides high-resolution results much more quickly, with a single or a few samples in hand.

“We need to think about detecting variants and dealing with this virus more like reconnaissance – mission-like reconnaissance – meaning that it's much more proactive” than the current surveillance approaches, says Sokurenko. “[If] you know where and when to expect the attack, you know what is the best way to contain it.”

A grant awarded through the National Institutes of Health is fueling the development of the test, which will also include a barcode component that can be scanned through a smartphone app. The app connects the user to a database of all variant fingerprints. Preprogrammed and battery-capable thermocyclers, developed by Seattle’s IEH Laboratories, will also be made available for distribution with test materials.

A rapid COVID-19 variant test at UW Medicine's Sokurenko Lab.

Sokurenko says the test is already available as a prototype in the form of a same-day to next-day service in his lab on a collaborative basis, while a wider distribution of the full kit for clinical epidemiologists and researchers could come by this winter. He says the method is also designed with a key ability: potentially tracking new variants as they emerge.

“With emergence of new variants, generally, what we see right now involves a new combination of the known mutations. So we will be able to detect with this test, [the] emergence of new combinations of mutations, meaning new variants.”

Zach Garcia, UW Medicine

Terms of appropriate usage of file downloads

  • News reporters and news organizations may freely republish and distribute videos, still images and audio files produced by UW Medicine and the University of Washington School of Medicine.
  • Works must be attributed/credited appropriately (for example, “UW Medicine” – as denoted in the file) and must not be used for commercial purposes.
  • These visual and audio files may not be used to exploit or misrepresent UW Medicine or the University of Washington.
  • UW Medicine often licenses still images from Thinkstock but cannot grant republishing rights. You may not republish single image files credited to Thinkstock.
  • Logos of UW Medicine and University of Washington Health Sciences schools may not be republished without explicit permission. Contact us by phone or email: 206.543.3620 or